Tuesday, January 7, 2014

Phylogenetic Tree

Phylogenetic tree based on our research

The Phylogenetic tree comparing Rosa acicularis (Wild Rose), Rosa chinensis (China Rose), and Primla Vulgaris (Primrose). We created this using Jalview and Uniprot.org.

 
We couldn't find a Red Rose on the database so we substituted a China Rose this may have influenced the results. Even though we may have changed our results from substituting the China Rose our tree matched the tree that we created based on the pollen morphology of the plants that we observed under the SEM. This phylogenetic tree is more detailed and shows that the Wild Rose is closer related to the common ancestor of the Primrose than the China Rose. we guessed that the Wild Rose and the Red Rose would be just as distant from the Primrose's common ancestor but this was proved wrong assuming that the China Rose is closely related to the Red Rose.

Compound Microscope

Our group made wet mounts of all three of pollen to see if pollen tube growth could occur. Sadly two of our pollen samples did not end up growing pollen tubes, but one of ours did. The wild rose had what we believe to be the start of a pollen tube but the tube did not continue growing it just stayed the same.

Photo of the Primrose Pollen 
Captured by Haven, Toby and Henry

This is a photo of the primrose pollen the circles that are touching the pollen are air bubble not pollen tubes growing from the pollen grain.

Photo of the Wild Rose
Captured By Henry, Toby and Haven 

You can sort of see at the very edge of the white circle the pollen tube beginning to grow off of the pollen. It looked almost as if the pollen tube had a little foot.

Leica Digital Photos

Photo taken by Toby, Haven and Henry

This is a photo of part of the stub that has the primrose pollen stuck to it. you can see the triangular shape that the pollen has.

 Photo taken by Henry, Toby and Haven

This is a photo taken of the section of the stub that has the wild rose sample on it, the wild rose pollen is smaller then the Primrose so you cannot see the specific shape of the pollen.



Photo taken by Haven, Henry and Toby

This photo is of the section of the stud that has the red rose pollen on it, like the Wild rose pollen the red rose pollen is small so you cannot identify the shape through this photo.